Figure 6

HBZ induced C/EBPα expression. Total RNA was extracted from control or HBZ-expressing Kit 225 (A) and CD4⁺ cells of HBZ transgenic mice (B). Real-time PCR was performed to analyze the expression of CEBPA mRNA. (C) HBZ activated transcription of the CEBPA promoter. 293T cells were transfected with the C/EBPα reporter plasmid with or without the HBZ-expressing plasmid. Luciferase activity was measured 48 hours after transfection. (D) HBZ binds to the C/EBPα promoter. After transfection with mycHis-HBZ and C/EBPα reporter vector for 48 hours, 293T cells were chromatin immunoprecipitated by anti–c-Myc antibody. The precipitated DNAs and 1% of the input cell lysates were amplified by the specific primers for CEBPA promoter.