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Figure 4 | Retrovirology

Figure 4

From: HTLV-1 bZIP factor supports proliferation of adult T cell leukemia cells through suppression of C/EBPα signaling

Figure 4

Domains of HBZ responsible for the suppression of C/EBPα signaling. (A) Schematic diagram of HBZ and its mutants used in this study. Characteristic domains of HBZ are indicated as follows: activation domain (AD), central domain (CD), and basic leucine zipper domain (bZIP). (B) Analysis of HBZ deletion mutants for their effect on C/EBPα-mediated signaling. Jurkat cells were cotransfected with pC/EBP-Luc, phRL-TK, pCMV-Tag-C/EBPα and pME18Sneo-HBZ mutants. After 48 hours, the cells were harvested and analyzed for luciferase activity. *P < 0.05; **P < 0.01. (C) Determination of the region of HBZ responsible for the interaction with C/EBPα. 293T cells were transfected with the indicated mycHis-HBZ mutants together with the FLAG-C/EBPα. Cell lysates were subjected to immunoprecipitation using anti–c-Myc followed by immunoblotting using anti-FLAG. (D) The schema of C/EBPα deletion mutants is shown. The locations of the AD domain and the bZIP domain are indicated. (E) Mapping the region of the C/EBPα protein necessary for interaction with HBZ. 293T cells were transfected with mycHis-HBZ and full-length or mutant FLAG-C/EBPα. At 48 hours after transfection, total cell lysates were subjected to IP using anti-FLAG followed by IB using anti–His.

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