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Figure 1 | Retrovirology

Figure 1

From: HTLV-1 bZIP factor supports proliferation of adult T cell leukemia cells through suppression of C/EBPα signaling

Figure 1

HBZ suppressed C/EBPα signaling. (A) HBZ repressed C/EBPα-induced transcriptional activation. Jurkat cells were cotransfected with pC/EBP-Luc (0.5 μg), phRL-TK (10 ng), pME18Sneo-HBZ (0, 0.5, 1, and 2 μg), and pCMV-Tag-C/EBPα (1 μg). After 48 hours, the cells were harvested and analyzed for luciferase activity. (B) HBZ impaired the suppressive effect of C/EBPα on HTLV-1 LTR activation. Jurkat cells were cotransfected with pLTR-Luc (0.5 μg), phRL-TK (10 ng), and pME18Sneo-HBZ (2 μg), pCG-Tax (1 μg), together with pCMV-Tag-C/EBPα (1 μg). At 48 hours after transfection, a dual luciferase reporter assay was performed. All the data shown are relative values of firefly luciferase normalized to Renilla luciferase and expressed as mean of a triplicate set of experiments ± SD. *P < 0.05; **P < 0.01.

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Retrovirology

ISSN: 1742-4690

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