Contribution of virus-associated PKA activity in endogenous reverse transcription. NL4.3 viruses were subjected to ERT experiments run in the presence of dNTPs and increasing concentrations of H89 (A) or Myr-PKI (B). For each reaction, nucleic acids were extracted and (-)ssDBNA production was quantified by qPCR amplification. DNA levels detected in ERT reactions performed in the presence of nevirapine are indicated by a dotted line. Data are mean values of duplicate experiments and are expressed as a percentage of WT conditions.