Figure 5

Human A3A, 3B, 3C and 3F proteins restrict ZfL2-2 retrotransposition. (A) Inhibitory effect of mouse and human A3 proteins on retrotransposition of hL1, ZfL2-2 and UnaL2 retrotransposons. HeLa cells were co-transfected with 1 μg of the indicated APOBEC effector plasmid and 1 μg of retrotransposon target plasmid. Relative retrotransposition efficiency was determined by counting the fixed and stained neomycin resistant colonies, formed after 12 days of G418 selection (Additional file 1: Figure S3), and calculated by setting the value obtained for control cells co-transfected with retrotransposon plasmid and an empty vector at 100%. Data are the means ± SD of at least three independent experiments. ***P < 0.001, **P < 0.01, *P < 0.05, t-test. (B) The expression of HA, Myc, or V5 epitope-tagged proteins tested in (A) was confirmed in the HEK293T model by immunoblotting. GAPDH protein levels were used as a loading control.