Figure 1

Siglec-7 binds recombinant env gp120. A, Dot plot flow cytometry graphs showing representative examples of negative and positive controls for the binding experiments reported in the 1B and 1C panels. The negative control graph (left) illustrates the absence of interactions between env gp120 from HIV-1 IIIB strain covalently conjugated to carboxyl microparticles and phycoerythrin (PE)-labeled goat anti-human (GAH). The positive control shows the binding of env gp120 from HIV-1 IIIB strain covalently conjugated to carboxyl microparticles to PE-labeled anti-env gp120 mAb (clone 2G12). B, Dot plot flow cytometry graphs showing a representative example of the binding of NKp44 (left) or Siglec-7 (right) Fc fusion proteins to env gp120 from HIV-1 IIIB strain covalently conjugated to carboxyl microparticles. The binding was detected trough a PE-labeled GAH Fc Ab conjugated to the fusion proteins. C, Dot plot flow cytometry graphs showing a representative example of the binding of Siglec-7 Fc fusion protein to env gp120 from HIV-1 IIIB strain covalently conjugated to carboxyl microparticles in the presence of a pool of anti Siglec-7-specifc antibodies (left) and of gp120 neuraminidase treatment (right).