Figure 3

The gp41 content and morphology of residual virions derived from peptide triazole treatments of HIV-1 BaL pseudovirus. (a) Reactivity of residual viruses, from 18.2-19% Optiprep fractions, to gp41 antibodies 2 F5 and 4E10 as a function of KR13 and HNG156 concentration. (b). Reactivity of residual viruses to gp41 antibodies as a function of time of treatment with KR13 and HNG156. All samples in (a) and (b) were normalized to total gp41 content on intact virion. Error bars represent the standard deviation of the mean, n = 3. (c) Average diameter of HIV-1 BaL virions untreated and post treatment with KR13 (1 μM) as determined by TEM. The probability distribution box plot shows the average in red and the distribution in bar lines (n = 16). The diameter analysis was conducted using Image J Software. Inset: Representative TEM Images obtained at designated time points, with the scale bar representing 50 nm diameter.