Dose response of the effects of peptide triazoles on HIV-1 BaL pseudovirus. (a) Inhibition of cell infection was measured using a single round infection assay. The EC50 values are reported in Table 1. (b) Relative gp120 shedding was determined for the peptides KR13, KR13b, HNG156 and KR13s using western blot analysis (see Methods section). Electrophoretic bands of gp120 in soluble protein and residual virus fractions were quantified using Image J analysis; IC50 values obtained are in Table 1. A low level of gp120 shedding (<5%) was observed with the intact virus used as the negative control. Data were normalized to 100% gp120 shedding observed with 1% triton X treated lysed virus. (c) Relative p24 release induced by peptides was measured using ELISA. The data were normalized using untreated virus as negative control (<5% p24 release), and p24 release observed with 1% triton X treated virus was taken as 100% p24 content. The p24 release IC50 value for KR13 was 500 ± 80 nM, while the other peptide triazoles exhibited no significant p24 leakage. Sigmoidal curve fits of data were obtained using Origin Pro.8 (Origin Lab). Error bars represent the standard deviation of the mean, n > 3.