Allosteric induction of the CD4-bound conformation of HIV-1 Gp120

Retrovirology

Table 2 Sequences of peptides isolated by screening the “X6 NNK” m2-based library against gp120 BaL and trimeric R2 gp140

The “X6 NNK” library was screened using standard conditions, stringent conditions or the complexed bait method, see Methods. Thousands of potential gp120 BaL binding clones and trimeric R2 gp140-binding clones were obtained of which more than a hundred were confirmed as envelope binders. A total of 75 clones were sequenced and the sequences of peptides which were isolated against both envelopes or isolated more than once are shown (the number of times each sequence was isolated for each envelope is given in the table). For convenience, the constant first and last cysteine residues, as well as residues of the X6 common motif, (xRxDLPxWAxxx) are shown in bold. Moreover, note the common Threonine at position 13.

ISSN: 1742-4690