Figure 3
From: Allosteric induction of the CD4-bound conformation of HIV-1 Gp120
The m2-phage binds monomeric and trimeric envelope proteins of different HIV-1 isolates. Equal amounts of phages and CD4 were applied to nitrocellulose filters and incubated with gp120 CDC451, gp120 BaL, trimeric R2 gp140 or without any envelope as indicated (no signals developed in the absence of envelope, not shown). Captured envelope proteins were detected using HIVIg or the CD4i mAb CG10.