Figure 8

The S40F mutation suppresses the block to budding imposed by CA[EE ₇₅ , ₇₆ AA] and CA[P ₉₉ A]. The samples described in the legend to Figure 7 were analyzed by electron microscopy. F. CA[P99A]-p6[P7L-S40F] Panel A, P7L; panel B, CA [EE_75,76AA]-p6[P₇L]; panel C, CA[P₉₉A]-p6[P₇L]; panel D, P7L-S40F; panel E, CA[EE_75,76AA]-p6[P₇L-S₄₀F]; and panel F, CA[P₉₉A]-p6[P₇L-S₄₀F].