Figure 6
The S40F mutation increases the disruption of virus release due to nonfunctional L domains-1 and −2. Panel A, COS-1 cells transfected with DNA encoding the indicated constructs were analyzed by Western blotting after preparation of VLPs and cell lysates. Panel B, Quantitative analysis of VLP release efficiency [VLP/(VLP + Gag from cell lysate)] were determined as described in Methods (n = 7; 3 independent constructs of P7L-S40F-Gag).