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Figure 5 | Retrovirology

Figure 5

From: The S40 residue in HIV-1 Gag p6 impacts local and distal budding determinants, revealing additional late domain activities

Figure 5

Disrupting Alix interaction with S40F restores spherical particle formation. COS-1 cells were transfected with plasmids expressing HA-tagged -P7L (panels A and D), P7L-S40F (panels B and E), or P7L-Y36S-S40F (panels C and F). Panels C-F, cells were prepared for examination by immunoelectron microscopy as described in Methods. Bars in panels A, and B measure 500 nm; bars in panels C, D and F measure 100 nm; bar in panel E measures 2000 nm.

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