Table 1 Comparison of viral infections of samhd1 KO and wild-type mice and derived cells [12, 13]
From: SAMHD1 knockout mice: modeling retrovirus restriction in vivo
| Â | Rehwinkel et al. [[12]] | Behrendt et al. [[13]] | |
|---|---|---|---|
In vitro HIV-1 vector infection of cells from samhd1 KO mice compared with wild-type mice | • No effect on the infectivity of HIV-1 vector (wild-type RT) at 1–2 dpi* 5-fold increase of HIV-1 RT mutant (V148I) vector infection in BM-DC, BM-DMs, and MEFs | • ~5-fold increased infectivity of HIV-1 vector (wild-type RT) in BM-DCs at 3 dpi* Likely dependent on HIV-1 vector strains | |
In vivo HIV-1 vector transduction of samhd1 KO mice compared with wild-type mice | Effects on HIV-1 vector transduction | • No effect on HIV-1 vector (wild-type RT) at 5–6 dpi* 3-8-fold increase of HIV-1 RT mutant (V148I) in multiple cell types at 5–6 dpi* | • ~4-fold increased HIV-1 vector transduction (wild-type RT) in multiple cell types at 3 dpi* |
HIV-1 viral vector used | • pRRLsin.eGFP/ pCMVΔ8.2 vector (encoding Vif, Vpr, Vpu, and Nef) HIV-1-based pCSGW/ p8.91 vector (no accessory genes) | • HIV-1NL4-3 based GFP reporter vector (pHR.CMVGFP/ pCMVDR8.91) | |
Amount of HIV-1 vector injected | • Intravenously with 5 × 107 or 1 × 108 293T cell infectious units | • Intravenously with 5 × 106 viral particles | |
Exogenous Mo-MLV replication | No effect | ND# | |
Friend virus infection (a type of MLV) | ND# | No effect | |
Endogenous Mo-MLV replication or mouse retrotransposons | No effect | ND# | |
Encephalomyocarditis virus infection (a mouse RNA virus that induces IFNα) | No effect | ND# | |