Figure 8
From: Identification of late assembly domains of the human endogenous retrovirus-K(HML-2)
Overexpression of Tsg101 and Alix rescue HERV-K(HML-2) defective L-domain mutants. HEK 293T cells were transfected with WT or oriHERV-K113 L-domain mutants with and without cotransfection of HA-Tsg101 or FLAG-Alix. A luciferase vector was added for normalization. RT activities in the supernatants were determined at 48 h post transfection and normalized. The adjusted RT activities are shown in relation to the WT. The means of three experiments performed in quadruplicate are shown with standard error bars. The quantity of DNA per transfection was held constant by addition of an empty pcDNA3.1 vector.