Figure 6
From: Identification of late assembly domains of the human endogenous retrovirus-K(HML-2)
The YPX n L1 and the YPX n L2 motifs function as additional L-domains. (A) Comparison of the release of various L-domain mutants from transfected HEK 293T cells with that of the wild type oriHERV-K113. Means and standard errors of a representative experiment performed in quadruplicates are shown. (B) SDS-PAGE of virus pellets from the supernatants of HEK 293T cells transfected with WT and L-domain mutants. The Western blots were probed with a rat-anti-p27 CA serum. The cell lysates were in addition hybridized with an anti-actin antibody. A representative experiment of four performed is shown. The star depicts an unspecific band. (C) EM analysis of YPXnL defective phenotypes in HEK 293T cells.