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Figure 5 | Retrovirology

Figure 5

From: Identification of late assembly domains of the human endogenous retrovirus-K(HML-2)

Figure 5

Tsg101 is recruited by the PTAP motif of HERV-K(HML-2) Gag to sites of particle egress. Cells were visualized by confocal microscopy as described in the material and methods. Scale bar is 20 μm. (A) HEK 293T cells were transfected either with a full-length HA-Tsg101 expression vector or with Gag-Cherry alone. (B) Colocalization of HA-Tsg101 and Gag-Cherry in cells expressing both proteins. (C) No colocalization is observable if GagPTAP--Cherry is expressed. (D) HA-Tsg-3′, a mutant lacking the UEV domain, also fails to colocalize with Gag-Cherry.

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