Figure 4
From: Identification of late assembly domains of the human endogenous retrovirus-K(HML-2)
Mutation of the PTAP motif impairs particle release and induces an L-domain phenotype. (A) Effect of PTAP inactivation on the release of viral particles in HEK 293T cells. Data represent the mean of four experiments with standard errors. (B) SDS PAGE of pelleted virions (left panel) and lysates of the producer cells (right panel). The Gag precursor and processed CA proteins in cell lysates were immunoprecipitated and detected by Western blotting. The lysate of the PTAP mutant contains more p74Gag precursor protein and less p27 CA. The p27 CA protein is presumably reminiscent of released mature viruses attached to the cells. (C) Thin section EM analysis of HEK 293T cells transfected with PTAP mutant oriHERV-K113.