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Figure 3 | Retrovirology

Figure 3

From: Identification of late assembly domains of the human endogenous retrovirus-K(HML-2)

Figure 3

Deletion of the QP1 and QP2 peptides do not affect HERV-K(HML-2) release. (A) Reverse transcriptase (RT) activity in the supernatants of cells expressing the wild type oriHERV-K113 and the QP1/2 deletion mutant (ΔQP). The RT activity was normalized by cotransfection of a luciferase expression plasmid. Data represent means of four samples with error bars (standard error) of a representative experiment. The activity with the wild type virus was set at 100%. (B) Released viral particles were pelleted by ultracentrifugation and subjected to SDS-PAGE. The Western blot was probed with a CA-specific polyclonal serum or an actin specific antibody. (C) Electron microscopic analysis of the WT and ΔQP mutant morphology. Electron micrographs show mature particles released from HEK 293T cells. No differences in the morphology of the particles were detectable.

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