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Table 2 Neutralization of Env glycosylation mutants by MAbs

From: A systematic study of the N-glycosylation sites of HIV-1 envelope protein on infectivity and antibody-mediated neutralization

Env Region MAbs/IC50 (μg/mL)(%)
Percentage of neutralization sensitivity relative to WT
2F5 4E10 b12 VRC03 VRC01 PG9 PG16 3869
FE(WT) - 2.224 (100) 2.986 (100) 0.088 (100) 2.601 (100) 5.500 (100) 1.415 (100) 7.600 (100) >25 (100)
N88D C1 1.097 (203) 1.840 (162) 0.201 (44) 3.427 (76) 4.936 (111) 0.988 (143) 12.847 (59) ND
N133Q V1/V2 1.571 (142) 1.776 (168) 0.123 (72) 2.962 (88) 5.178 (106) 2.479 (57) 16.233 (47) 5.035 (>496)
N142Q V1/V2 1.117 (199) 0.999 (299) 0.035 (251) 2.944 (88) 5.100 (108) 3.041 (47) 16.350 (46) 1.315 (>1901)
N197D V1/V2 0.396 (562) 0.477 (626) 0.005 (1760) 0.070 (3716) 2.355 (234) 16.123 (9) >25 (<30) 5.540 (>451)
N289D C2 1.250 (178) 1.544 (193) 0.150 (59) 2.539 (102) 3.800 (145) 0.519 (273) 0.731 (1040) ND
N301Q V3 0.210 (1059) 0.585 (510) 0.040 (220) 1.235 (211) 2.330 (236) 0.875 (162) 14.884 (51) ND
N339Q C3 1.127 (197) 1.579 (189) 0.083 (106) 1.799 (145) 4.899 (112) 1.664 (85) 14.644 (52) ND
N355Q C3 0.426 (522) 0.353 (846) 0.060 (147) 1.980 (131) 5.456 (101) 0.800 (177) 15.250 (50) ND
N392Q V4 1.265 (176) 2.327 (128) 0.051 (173) 4.908 (53) 5.678 (97) 1.091 (130) 14.064 (54) ND
N408Q V4 1.505 (148) 2.010 (149) 0.063 (140) 2.938 (89) 5.777 (95) 1.775 (80) 16.512 (46) ND
N411Q V4 1.072 (207) 1.302 (229) 0.082 (107) 3.511 (74) 5.230 (105) 0.900 (157) 14.783 (51) ND
N442Q C4 0.781 (285) 0.934 (320) 0.045 (196) 0.791 (329) 4.120 (133) 1.650 (86) 16.111 (47) ND
N448Q C4 0.635 (350) 0.694 (430) 0.057 (154) 2.917 (89) 4.987 (110) 0.878 (161) 13.386 (57) ND
N463Q V5 0.763 (291) 0.895 (334) 0.056 (157) 2.170 (120) 3.450 (159) 2.544 (56) 16.333 (47) ND
N466Q V5 1.339 (166) 1.397 (214) 0.087 (101) 2.139 (122) 3.204 (172) 3.070 (46) 16.378 (46) ND
N611Q gp41 1.836 (121) 0.704 (424) 0.116 (76) 3.103 (84) 5.431 (101) 1.797 (79) 15.830 (48) ND
N625Q gp41 0.400 (556) 0.577 (518) 0.041 (215) 3.329 (78) 5.398 (102) 1.069 (132) 16.450 (46) ND
N637Q gp41 1.429 (156) 1.168 (256) 0.102 (86) 2.812 (92) 5.178 (106) 1.342 (105) 13.411 (57) ND
  1. Percentage of neutralization sensitivity relative to WT (%) = IC50 WT / IC50 mutant.
  2. ND: not detected.