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Table 2 Neutralization of Env glycosylation mutants by MAbs

From: A systematic study of the N-glycosylation sites of HIV-1 envelope protein on infectivity and antibody-mediated neutralization

Env

Region

MAbs/IC50 (μg/mL)(%)

Percentage of neutralization sensitivity relative to WT

2F5

4E10

b12

VRC03

VRC01

PG9

PG16

3869

FE(WT)

-

2.224 (100)

2.986 (100)

0.088 (100)

2.601 (100)

5.500 (100)

1.415 (100)

7.600 (100)

>25 (100)

N88D

C1

1.097 (203)

1.840 (162)

0.201 (44)

3.427 (76)

4.936 (111)

0.988 (143)

12.847 (59)

ND

N133Q

V1/V2

1.571 (142)

1.776 (168)

0.123 (72)

2.962 (88)

5.178 (106)

2.479 (57)

16.233 (47)

5.035 (>496)

N142Q

V1/V2

1.117 (199)

0.999 (299)

0.035 (251)

2.944 (88)

5.100 (108)

3.041 (47)

16.350 (46)

1.315 (>1901)

N197D

V1/V2

0.396 (562)

0.477 (626)

0.005 (1760)

0.070 (3716)

2.355 (234)

16.123 (9)

>25 (<30)

5.540 (>451)

N289D

C2

1.250 (178)

1.544 (193)

0.150 (59)

2.539 (102)

3.800 (145)

0.519 (273)

0.731 (1040)

ND

N301Q

V3

0.210 (1059)

0.585 (510)

0.040 (220)

1.235 (211)

2.330 (236)

0.875 (162)

14.884 (51)

ND

N339Q

C3

1.127 (197)

1.579 (189)

0.083 (106)

1.799 (145)

4.899 (112)

1.664 (85)

14.644 (52)

ND

N355Q

C3

0.426 (522)

0.353 (846)

0.060 (147)

1.980 (131)

5.456 (101)

0.800 (177)

15.250 (50)

ND

N392Q

V4

1.265 (176)

2.327 (128)

0.051 (173)

4.908 (53)

5.678 (97)

1.091 (130)

14.064 (54)

ND

N408Q

V4

1.505 (148)

2.010 (149)

0.063 (140)

2.938 (89)

5.777 (95)

1.775 (80)

16.512 (46)

ND

N411Q

V4

1.072 (207)

1.302 (229)

0.082 (107)

3.511 (74)

5.230 (105)

0.900 (157)

14.783 (51)

ND

N442Q

C4

0.781 (285)

0.934 (320)

0.045 (196)

0.791 (329)

4.120 (133)

1.650 (86)

16.111 (47)

ND

N448Q

C4

0.635 (350)

0.694 (430)

0.057 (154)

2.917 (89)

4.987 (110)

0.878 (161)

13.386 (57)

ND

N463Q

V5

0.763 (291)

0.895 (334)

0.056 (157)

2.170 (120)

3.450 (159)

2.544 (56)

16.333 (47)

ND

N466Q

V5

1.339 (166)

1.397 (214)

0.087 (101)

2.139 (122)

3.204 (172)

3.070 (46)

16.378 (46)

ND

N611Q

gp41

1.836 (121)

0.704 (424)

0.116 (76)

3.103 (84)

5.431 (101)

1.797 (79)

15.830 (48)

ND

N625Q

gp41

0.400 (556)

0.577 (518)

0.041 (215)

3.329 (78)

5.398 (102)

1.069 (132)

16.450 (46)

ND

N637Q

gp41

1.429 (156)

1.168 (256)

0.102 (86)

2.812 (92)

5.178 (106)

1.342 (105)

13.411 (57)

ND

  1. Percentage of neutralization sensitivity relative to WT (%) = IC50 WT / IC50 mutant.
  2. ND: not detected.
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