Primate lentiviral Nef proteins deregulate T-cell development by multiple mechanisms

Retrovirology

Table 2 Artificial Nef mutants used

	CD3	CD4	CXCR4	MHC-I	PAK2	TGR	Reference
HIV-1	-	+	+/-	+
B2 VGF-AAA	- *	+ *	- *	- *	ND	2.5 *	This study
NA7 VGF-AAA	- *	+	-	-	-	3.7 *	[29]
C1422 VGF-AAA	- *	+ *	- *	- *	ND	1.7 *	This study
NA7 F191H	-	+	- *	+	+/-	1.0 *	[35]
NA7 F191R	-	+	- *	+	-	1.7 *	[35]
SF2 F195I	- *	+	- *	+	+/-	0.3 *	[36]
SF2 F195A	- *	+	- *	+	-	0.7 *	[37]
SIV		+	++	+
mac AXXP	++ *	+	+/- *	+	+ *	0.3 *	[38]
mac AXXA	++	+	- *	+	+/- *	0.2 *	[38, 39]
mac PXXA	++ *	+	- *	+	+/- *	0.2 *	[38]
mac H121R	++ *	+ *	- *	- *	+/- *	0.3 *	This study
mac Y221R	++ *	+ *	- *	- *	+/- *	0.6 *	This study
mac H121R/Y221R	++ *	+ *	- *	- *	- *	0.5 *	This study
mac ∆153	++	-	- *	-	- *	0.2 *	[39]
mac ∆183	-	-	- *	-	- *	1.2 *	[39]
blu RR-AA	-	+ *	+ *	+/-*	- *	0.5 *	[40]

Table shows properties of the mutated Nef proteins (grouped according to origin) used in this study, with reference to published data wherever possible: *indicates this study is the first report. Downregulation of cell surface markers by Nef in transduced CD4⁺ PBLs as indicated in column heading is reported semi-quantitatively: from – (no downregulation), over +/- and + to ++ (strong downregulation). PAK2: binding to PAK2 semi-quantitatively: from – (no detectable binding), over +/- to + (strong binding similar to corresponding wild-type). In boldface the expected phenotype for Nef from HIV-1, HIV-2 and SIV origin, when stereotypical. TGR: thymocyte generation ratio, control value is typically about 1.4. ND: not determined.

ISSN: 1742-4690