Skip to main content

Table 2 Artificial Nef mutants used

From: Primate lentiviral Nef proteins deregulate T-cell development by multiple mechanisms

  CD3 CD4 CXCR4 MHC-I PAK2 TGR Reference
HIV-1 - + +/- +    
B2 VGF-AAA - * + * - * - * ND 2.5 * This study
NA7 VGF-AAA - * + - - - 3.7 * [29]
C1422 VGF-AAA - * + * - * - * ND 1.7 * This study
NA7 F191H - + - * + +/- 1.0 * [35]
NA7 F191R - + - * + - 1.7 * [35]
SF2 F195I - * + - * + +/- 0.3 * [36]
SF2 F195A - * + - * + - 0.7 * [37]
SIV   + ++ +    
mac AXXP ++ * + +/- * + + * 0.3 * [38]
mac AXXA ++ + - * + +/- * 0.2 * [38, 39]
mac PXXA ++ * + - * + +/- * 0.2 * [38]
mac H121R ++ * + * - * - * +/- * 0.3 * This study
mac Y221R ++ * + * - * - * +/- * 0.6 * This study
mac H121R/Y221R ++ * + * - * - * - * 0.5 * This study
mac ∆153 ++ - - * - - * 0.2 * [39]
mac ∆183 - - - * - - * 1.2 * [39]
blu RR-AA - + * + * +/-* - * 0.5 * [40]
  1. Table shows properties of the mutated Nef proteins (grouped according to origin) used in this study, with reference to published data wherever possible: *indicates this study is the first report. Downregulation of cell surface markers by Nef in transduced CD4+ PBLs as indicated in column heading is reported semi-quantitatively: from – (no downregulation), over +/- and + to ++ (strong downregulation). PAK2: binding to PAK2 semi-quantitatively: from – (no detectable binding), over +/- to + (strong binding similar to corresponding wild-type). In boldface the expected phenotype for Nef from HIV-1, HIV-2 and SIV origin, when stereotypical. TGR: thymocyte generation ratio, control value is typically about 1.4. ND: not determined.