Primate lentiviral Nef proteins deregulate T-cell development by multiple mechanisms

Retrovirology

Table 1 Natural Nef variants used

	CD3	CD4	CXCR4	MHC-I	PAK2	TGR	References
HIV-1	-	+	+/-	+	+
B2	- *	+	+/-	+	ND	0.4 *	[29]
B10	- *	+	+/-	+	ND	0.4 *	[29]
B2681	- *	+	+/-	+	ND	0.6 *	[29]
BA-L	-	+	+/-	+	ND	0.6 *	[29]
NA7	-	+	+/-	+	+/-	0.4	[25, 29, 31]
SF2	-	+	+/-	+	+	0.1 *	[29, 31]
C794	- *	+	+/-	-	ND	0.6 *	[29]
C1044	- *	+	+/-	-	ND	0.2 *	[29]
C1422	- *	+	+/-	+	ND	0.2 *	[29]
O4	- *	+	+/-	+	ND	0.1 *	[29]
O8	- *	+	-	-	ND	1.6 *	[29]
O14	- *	+	+/-	+	ND	0.2 *	[29]
HIV-2	++	+	+	+
EHO	++*	+*	+*	+*	ND	0.1 *	[32]
171	+ *	+	-	+/-	ND	0.6 *	[29]
SIV		+	++	+
cpz	-	+	+	+	+	1.0 *	[28, 31]
gor	-	+	++	+	ND	0.3 *	[33]
mac	++	+	++	+	+	0.1 *	[28, 31, 34]
blu	++	+	+	+	+/-	0.2 *	[28, 31]
smm	++	+	++	+	+	0.3 *	[28, 31, 34]

Table shows properties of the Nef proteins (grouped according to origin) used in this study, with reference to published data wherever possible: *indicates this study is the first report. Downregulation of cell surface markers by Nef in transduced PBLs as indicated in column heading is reported semi-quantitatively: from – (no downregulation), over +/- and + to ++ (strong downregulation). PAK2: binding to PAK2 semi-quantitatively: from – (no detectable binding), over +/- to + (strong binding similar to corresponding wild-type). In boldface the expected phenotype for Nef from HIV-1, HIV-2 and SIV origin, when stereotypical. TGR: thymocyte generation ratio, average value is shown, control value is typically about 1.4.

ISSN: 1742-4690