Table 2 Antiviral activity (IC 50 ) of i & i + 7 stapled peptides against HIV-1 subtype A, A/D, A2/D, AG, B, C and D ENV pseudotyped reference viruses
From: Dual-acting stapled peptides target both HIV-1 entry and assembly
NIH # | ENV clone | Subtype | NYAD-36 | NYAD-66 | NYAD-67 |
|---|---|---|---|---|---|
IC50(μM) | IC50(μM) | IC50(μM) | |||
11887 | Q259env.w6 | A | 3.5 ± 0.1 | 0.97 ± 0.04 | 0.22 ± 0.02 |
11891 | QF495.23M.ENV.A3 | A | 3.9 ± 0.3 | 3.1 ± 0.26 | 1.6 ± 0.06 |
11892 | QF495.23M.ENV.B2 | A | 4 ± 0.7 | 1.8 ± 0.2 | 0.9 ± 0.1 |
11894 | QG984.21M.ENV.A3 | A | 8.4 ± 0.5 | 1.9 ± 0.2 | 0.059 ± 0.01 |
11896 | QH343.21M.ENV.A10 | A | 3.6 ± 0.2 | 0.38 ± 0.03 | 0.56 ± 0.08 |
11897 | QH343.21M.ENV.B5 | A | 2 ± 0.25 | 2.2 ± 0.1 | 0.59 ± 0.09 |
11901 | QA790.204I.ENV.A4 | A/D | 1.3 ± 0.3 | 0.63 ± 0.06 | 0.22 ± 0.02 |
11903 | QA790.204I.ENV.C8 | A/D | 2 ± 0.3 | 1.2 ± 0.2 | 0.36 ± 0.06 |
11904 | QA790.204I.ENV.E2 | A/D | 1.9 ± 0.1 | 1 ± 0.1 | 0.47 ± 0.04 |
11905 | QG393.60M.ENV.A1 | A2/D | 6.5 ± 1.2 | 1.1 ± 0.22 | 1.1 ± 0.11 |
11906 | QG393.60M.ENV.B7 | A2D | ~10.8 | 9 ± 0.9 | 2.1 ± 0.2 |
11907 | QG393.60M.ENV.B8 | A2/D | 7.9 ± 0.6 | 10.4 ± 0.6 | 1.8 ± 0.06 |
11591 | CRF02_AG Clone 211 | AG | 9.4 ± 0.2 | 15.3 ± 0.7 | 1.8 ± 0.05 |
11595 | CRF02-AG Clone 251 | AG | 4.1 ± 0.3 | 1.1 ± 0.08 | 0.95 ± 0.03 |
11599 | CRF02-AG Clone 257 | AG | 3.9 ± 0.17 | 2.2 ± 0.26 | 0.62 ± 0.02 |
11604 | CRF02_AG Clone 271 | AG | 2 ± 0.05 | 3 ± 0.2 | 0.15 ± 0.01 |
11022 | PVO, clone 4 (SVPB11) | B | 1.6 ± 0.2 | 0.43 ± 0.08 | 0.26 ± 0.006 |
11023 | TRO, Clone 11 (SVPB12) | B | 7.7 ± 0.8 | >14 | 2.3 ± 0.2 |
11035 | pREJO4541 clone 67 (SVPB16) | B | 5.7 ± 0.6 | 8.2 ± 0.2 | 0.84 ± 0.03 |
11036 | pRHPA4259 clone 7 (SVPB14) | B | 3.1 ± 0.3 | 0.66 ± 0.17 | 0.42 ± 0.1 |
11037 | pTHRO4156 clone 18 (SVPB15) | B | 3.6 ± 0.32 | 1.7 ± 0.15 | 0.48 ± 0.01 |
11038 | pCAAN5342 clone A2 (SVPB19) | B | 4.8 ± 0.6 | 0.77 ± 0.13 | 0.47 ± 0.06 |
11058 | SC422661, clone B (SVPB8) | B | 7.1 ± 0.5 | 3.8 ± 0.2 | 1.8 ± 0.1 |
11507 | HIV-25925-2, clone 22 | C | 6.8 ± 0.3 | 1.3 ± 0.15 | 1 ± 0.13 |
11508 | HIV-26191-2, clone 48 | C | 4.5 ± 0.3 | 1.5 ± 0.4 | 2.6 ± 0.5 |
11307 | Du172.17 | C | 3.5 ± 0.15 | 1.8 ± 0.1 | 0.47 ± 0.02 |
11308 | Du422.1 | C | 2.3 ± 0.25 | 1.3 ± 0.2 | 1.1 ± 0.11 |
11310 | ZM214M.PL15 | C | 1.9 ± 0.1 | 1 ± 0.06 | 0.39 ± 0.02 |
11313 | ZM53M.PB12 | C | 1.7 ± 0.05 | 1.1 ± 0.06 | 1.6 ± 0.2 |
11314 | ZM109F.PB4 | C | 3.3 ± 0.2 | 3.2 ± 0.2 | 0.7 ± 0.1 |
11317 | CAP210.2.00.E8 | C | 3.6 ± 0.05 | 2.5 ± 0.1 | 0.9 ± 0.09 |
11911 | QA013.70I.ENV.H1 | D | 3.5 ± 0.2 | 1.4 ± 0.3 | 0.48 ± 0.06 |
11912 | QA013.70I.ENV.M12 | D | 3.4 ± 0.1 | 2 ± 0.2 | 0.45 ± 0.02 |
11913 | QA465.59M.ENV.A1 | D | 1.8 ± 0.3 | 1.2 ± 0.1 | 2 ± 0.3 |
11914 | QA465.59M.ENV.D1 | D | 0.93 ± 0.1 | 0.98 ± 0.02 | 1.2 ± 0.06 |
11916 | QD435.100M.ENV.B5 | D | 10.1 ± 1.9 | 2 ± 0.1 | 0.3 ± 0.03 |