Figure 7
From: Dual-acting stapled peptides target both HIV-1 entry and assembly
HIV-1 particles produced in the presence of stapled peptides show defects in infectivity. Six hours posttransfection with pNL4-3, 293T cells were treated with indicated concentrations of NYAD-36, -66, and -67 and virions were collected after 2 days. RT-normalized virus stocks were used to infect TZM-bl cells for 2 h in a total volume of 100 μl (the concentration of peptides from producer cells was diluted ~20-fold). Two days after infection, cells were washed, lysed and assayed for luciferase activity. N = 8, ± SD.