Figure 6

i + 7 stapled peptides have no effect on HIV-1 release, but impair Gag processing. (a) 293T cells were transfected with pNL4-3 and 6 h after transfection treated with indicated concentrations of NYAD-36, -66, and -67 for 16–20 h. Cells were metabolically labeled with [³⁵S]Met/Cys for 2 h. Cells were lysed and virions were collected by ultracentrifugation. Cell and virus lysates were immunoprecipitated with HIV-Ig and subjected to SDS-PAGE. Protein band intensities were quantified by phosphorimager analysis, and HIV-1 release was calculated as the amount of virion-associated p24 relative to total (cell- plus virion-associated) Gag. (b) Accumulation of unprocessed Gag in cells was measured by calculating the ratio of Pr55^Gag to p24 in cells. P values were calculated by Student’s t-test, with P < 0.05 considered significant. N = 3, ± SD.