Figure 2

Cell penetration of staple peptides in 293T cells. FACS analyses of 293T cells incubated with 4 μM FAM-conjugated peptides for 20 h at 37°C. The cells were treated with 0.25% Trypsin-EDTA for 30 min at 37°C and washed twice with 1x PBS before and after Trypsin-EDTA treatment. The experiment was repeated twice with similar results. The mean fluorescence intensity (MFI) was used to quantify the cellular uptake of FAM-conjugated peptides. NYAD-1 was used as positive control and a FAM-conjugated linear peptide, NYAD-41, was used as a negative control.