Figure 13
From: Dual-acting stapled peptides target both HIV-1 entry and assembly
VSV-G pseudotyped HIV-1 particles prepared in the presence of stapled peptides are also defective in single-cycle infectivity. 293T cells were cotransfected with an Env-defective pNL4-3 derivative (pNL4-3/KFS) and vectors expressing WT or V120Q/A327P Env, or VSV-G expression vector. Six hours after transfection cells were treated with 50 μM of stapled peptides, and virions were collected after 2 days. TZM-bl cells were infected with RT-normalized virus and infectivity was measured as described in legends to Figure 7. N = 6, ± SD.