Figure 2

Nef activates Hck-YEEI in the same manner as Csk-downregulated wild-type Hck in yeast. Yeast cultures were co-transformed with expression plasmids for wild-type Hck, Hck-YEEI, Csk, and Nef in the combinations shown. A) Cultures were grown on galactose-agar plates and scanned as described in the legend to Figure 1. B) Immunoblots from cultures shown in panel A. Transformed cells were grown in liquid culture in the presence of galactose at 30°C for 18 h. Protein extracts were separated via SDS-PAGE, and immunoblotted for tyrosine-phosphorylated proteins (pTyr) and for Hck, Csk, and Nef. Note that the numbers in panel A correspond with the lanes in panel B .