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Figure 10 | Retrovirology

Figure 10

From: Discovery of a diaminoquinoxaline benzenesulfonamide antagonist of HIV-1 Nef function using a yeast-based phenotypic screen

Figure 10

Comparison of DQBS with other Nef inhibitors on HIV replication and infectivity. A) Structures of the Nef inhibitors B9 and DLC27-14 (see main text for details). B) HIV-1 replication assays were performed in U87MG/CD4/CXCR4 cells in the absence (DMSO control) or presence of each compound as described in the legend to Figure 5. Cells infected with an equivalent p24 input of Nef-defective HIV (ΔNef) are included as a reference control. C) TZM-bl reporter cells, in which the HIV-1 LTR drives transcription of luciferase [56], were infected with wild-type or Nef-defective (ΔNef) HIV-1 NL4-3 in the absence (DMSO) or presence of each of the Nef inhibitors shown. Viral infectivity was assessed as luciferase activity 48 h later. This experiment was repeated in triplicate and the data are presented as mean percent infectivity relative to the DMSO control.

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