Figure 6

Reduced use of GPR15 by SIV Envs pre-triggered with sCD4. Pseudotype viruses carrying CD4-dependent or CD4-independent Envs were incubated with or without sCD4 (50 ug/ml for 1 hour) and assessed for their ability to infect cells 293 T cells expressing rmCCR5 and rmCD4 (A), rmCCR5 without CD4 (B), or rmGPR15 and rmCD4 (C). Each Env is indicated as a box (without sCD4) and a triangle (with sCD4), and the two conditions for each individual Env are connected by a line. Paired T-tests were used to compare entry with and without sCD4 incubation.