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Figure 1 | Retrovirology

Figure 1

From: Transcription elongation regulator 1 (TCERG1) regulates competent RNA polymerase II-mediated elongation of HIV-1 transcription and facilitates efficient viral replication

Figure 1

TCERG1 depletion decreases basal and Tat-activated transcription from the HIV-1 LTR. A. PBLs were co-transfected with a luciferase expression vector under the control of the HIV-1 LTR promoter (LTR-LUC) along with pGeneClip-shTCERG1-C1 or pGeneClip-shTCERG1-3 and pGeneClip-shTCERG1-4, and the latter pair together with pGeneClip-shTCERG1-1. The luciferase activity was quantified 72 hours after transfection as relative light units (RLUs) using the control cells as the basal signal. Data from three independent experiments are represented in the histogram (means ± SEM). Statistical analysis was performed and p-values are shown (**, p < 0.01; ***, p < 0.001). TCERG1 interference was determined by immunoblotting. Appropriate expression and nuclear localization of Tat was evaluated by immunofluorescence using a specific antibody against Tat and Dapi to stain the nucleus (a representative cell is shown). B. Jurkat cells with stable interference of TCERG1 were co-transfected with a luciferase expression vector under the control of the HIV-1 LTR promoter (LTR-LUC) along with CMV-Tat101 or pcDNA3 as negative control. The luciferase activity was quantified 72 hours after transfection as relative light units (RLUs) using the control cells as the basal signal. The results of three different experiments are represented in the histogram (means ± SEM). Statistical analysis was performed, and p-values are shown (**, p < 0.01; ***, p < 0.001). TCERG1 interference was determined by immunoblotting. The effect of TCERG1 interference on the expression and nuclear localization of Tat was analyzed by immunofluorescence in all Jurkat cell lines with stable interference of TCERG1.

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