Figure 2

ELISA-based CD85j/S100A8 and CD85j/S100A9 binding assay. (A) Anti-GST mAbs well-coated microtiter plates were incubated with 4 μg/mL of recombinant GST-tagged human S100A8 or S100A9 proteins followed by incubation with increasing amounts of CD85j-Fc protein. (B) CD85j-Fc protein well-coated microtiter plates were incubated with increasing concentrations of recombinant GST-tagged human S100A8 or S100A9 proteins. (C) CD85j-Fc protein well-coated were incubated with 4 μg/mL of human S100A8 or S100A9 proteins in the presence of increasing amounts of soluble CD85j-Fc protein. (D) CD85j-Fc protein well-coated were incubated with 4 μg/mL of human S100A8 or S100A9 proteins in the presence of increasing concentrations of soluble NKG2D-Fc protein. Each point is the mean ± SE of 4 determinations.