HIV-1 replication and CD4+T cell depletion in TOM. A) TOM (n = 8) were parenterally exposed to HIV-1 and the viral load monitored in PB plasma (black circles). Changes in CD4+ T cell levels in PB were measured over time using flow cytometric analysis (gray squares). Gating scheme: live cells → hCD45 → hCD3 → CD4. The limit of detection for viral load is indicated with a dashed line. B) The percentage of CD4+ T cells present in the tissues of infected (gray bars) and non-infected (black bars) TOM were analyzed using flow cytometric analysis. Gating scheme: live cells → hCD45 → hCD3 → CD4. C) HIV infection results in a reduction in the levels of effector memory cells within the CD4+ T cell population of infected mice (n = 5, grey bars) versus uninfected (n = 7, black bars) mice. Infected animals were approximately 48 weeks post-implantation at the time of harvest. For all graphs, error bars represent Mean ± SEM.