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Figure 3 | Retrovirology

Figure 3

From: Reactivation of latent HIV-1 in central memory CD4+T cells through TLR-1/2 stimulation

Figure 3

Signaling triggered by Pam3CSK4 in cultured T CM . Culture TCM were either left unstimulated (0 h) or stimulated with IL-2, Pam3CSK4 and αCD3/αCD28 after 30 minutes or 3 hours. Nuclear and cytoplasmic fractions were isolated as indicated in the experimental procedure section. Proteins were loaded on a SDS-polyacrylamide gel, transferred to a membrane and western-blotted against p50, p65, NFATc1, NFATc2, α-tubulin, histone H3 and β-actin (A) or against cJun, cFos, histone H3 and β-actin (B). Data is representative of two donors. (C) Culture TCM were left unstimulated (0 h) or stimulated with IL-2, Pam3CSK4 and αCD3/αCD28 after 30 minutes or 3 hours. Whole cell extracts were isolated as indicated in the experimental procedure section. Proteins were loaded on a SDS-polyacrylamide gel, transferred to a membrane and western-blotted against JNK, phospho-JNK, ERK, phospho-ERK and β-actin. Data is representative of two donors.

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