Figure 2

HERV-K(HML-2) expression is immediately down-regulated during embryoid body differentiation. A) Semi-quantitative RT-PCR of HERV-K(HML-2) expression in hFib2- iPS5 cells using the primer pair K21 and K7. The faint band at ≈570 bp presumably represents the rec mRNA of provirus ERVK-17 (see Table 1) which contains a small deletion in the amplified region. LMNA was amplified using an alternative primer (see Additional file 1 “Materials and Methods”) B) Quantitative expression analysis of HERV-K(HML-2) env, rec and Np9 transcripts in H9 cells. OCT4, LMNA, and ACTB serve as control [19, 20]. RNA levels are depicted relative to undifferentiated cells on day 0 (n=2, error bars +/-s.e.m).