Figure 7
Priming assay. (A) Amplifications from the products of RTion primed through mutant tRNALys3s targeting the TAR. +, heat-inactiviation of HIV-1-based vectors before infection; –, no heat-inactiviation. (B) Amplifications from the products of HIV-1 RTion primed through mutant tRNALys3s targeting the IN-encoding region and Env gene respectively. CTRL, CEM-SS cells were infected with heat-inactivated HIV-1-based vector and RTion products were amplified using the combined primers for detection of both types of the RTion products.