Figure 2

Elevated expression of schlafen 11 in HIV-1 elite controllers. (A) schlafen 11 (SLFN11) expression in unfractionated CD4+ T cells across disease states. Data points involved in subsequent protein characterization are highlighted with black bordering. (B) SLFN11 expression in central memory CD4+ T cells. (C) SLFN11 expression in effector memory CD4+ T cells. Reported p-values in panels A-C were obtained using unpaired t tests. (D) SLFN11 protein expression in elite controllers and non-controllers, as determined by western blot. Immunoblotting bands were quantified with ImageJ64 software. The quantified SLFN11 protein expression levels were normalized to corresponding GAPDH protein levels to ensure equal loading. (E) Correlation between SLFN11 normalized protein level and mRNA relative copy number. A Spearman’s rank test was used to evaluate the significance of the correlation.