Figure 3

Lower probability of glycosylation at position 332 correlates with the induction of cross-reactive neutralizing activity. (A) Pie charts representing the distribution of NLS and NIS PNGS motifs at position 332 between 12 individuals who developed and 9 individuals who did not develop CrNA. Red and green represent NLS and NIS motifs, respectively. (B) Residual virus infectivity in the presence of high concentrations of 2G12 (25 μg/ml), excluding viruses lacking one or more of the essential 2G12 glycans 295, 332, 386 or 392 [61, 62, 85], for virus clones that have NLS or NIS motif. (C) Residual virus infectivity in the presence of high concentrations of PGT126 (5 μg/ml), for virus clones that have NLS or NIS motifs. Values in the gray area are considered sensitive, while values in the white area are considered resistant.