Figure 3

LFA-1 dependency in ADAP-induced HIV-1 infection. (A) Single-cycle luciferase reporter HIV-1 was incubated for 2 hrs at 37 degree with C8166 cells that over-expressed GFP control or ADAP/GFP or M12/GFP, in the absence or presence of soluble ICAM-1-Fc or anti-CD18 treatment. After washing, these C8166 cells were further cultured for 72 hrs with or without soluble ICAM-1-Fc or anti-CD18. The amount of HIV-1 was measured by qRT-PCR using primers specific for the HIV-1 gag mRNA relative to an actin housekeeping gene (left panel) or was determined by luciferase assay (right panel). (B) Overexpression of ADAP or M12 in C8166 cells did not alter the surface expression levels of LFA-1. (C) Low (1 ng p24^Gag) or high (5 ng p24^Gag) amount of single-cycle luciferase reporter HIV-1 viruses were used to infect C8166 cells over-expressing GFP control or ADAP/GFP or M12/GFP. The luciferase readings were collected from triplicate samples with error bars (* represent p = <0.05, ** represents p = <0.01, *** represents p = <0.001).