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Figure 1 | Retrovirology

Figure 1

From: Immune adaptor ADAP in T cells regulates HIV-1 transcription and cell-cell viral spread via different co-receptors

Figure 1

Disruption the SLP-76-ADAP signaling module inhibits HIV-1 infection in T-cells. (A) Jurkat T cells were stably transfected with GFP, ADAP/GFP or M12/GFP and infected with single-cycle luciferase reporter HIV-1 (equivalent to 5 ng p24Gag/106 cells). The HIV-1 gag mRNA levels were determined by qRT-PCR 72 hrs post infection (P = 0.032, left and P = 0.005, right). The ADAP/M12 expression levels were examined by immunoblotting. (B) Overexpression of ADAP/GFP or M12/GFP in Jurkat cells did not alter the surface expression levels of CD4, CXCR4, CD3, CD28, β1 or β2 integrins as determined by flow cytometry. (C) Human primary CD4+ T cells were transfected with the scramble siRNA or siRNA targeting ADAP, and the knockdown efficiency was confirmed by immunoblotting (48 hrs post-transfection) or by qRT-PCR at different time points during HIV-1 infection. (D) Human primary CD4+ T cells were transfected with siRNAs for 24 hrs, then infected with single-cycle luciferase reporter HIV-1. The HIV-1 gag mRNA levels were determined by qRT-PCR 72 hrs post infection (left panel, P = 0.0192). Alternatively, the amount of HIV-1 was measured according to the luciferase readings (right panel, P = 0.0032) (* represents p = <0.05, ** represents p = <0.01).

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