Inter-subtype comparison of Nef-mediated CD4 and HLA-I downregulation capacities. Panels A and B : CD4 (A) and HLA-A*02 (B) downregulation activities of subtype A (red), B (orange), C (green) and D (blue) patient-derived Nef clones are shown. Bars represent the median and whiskers represent the inter-quartile range for each group. Kruskal-Wallis with Dunn’s multiple comparisons post-hoc tests were used to compare Nef functions between subtypes. The Kruskal-Wallis p-value is shown. In addition, significant differences between individual groups are indicated by asterisks above the bar indicating the two groups compared. The number of asterisks denotes the level of significance, namely, p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***). Panel C : CD4 and HLA-I downregulation functions within each subtype displayed significant positive relationships (Spearman’s, all p < 0.01).