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Figure 2 | Retrovirology

Figure 2

From: Ability of HIV-1 Nef to downregulate CD4 and HLA class I differs among viral subtypes

Figure 2

Function and Nef expression of patient-derived HIV-1 Nef clones. Panel A : Representative flow cytometry plots depict expression of surface CD4 (y-axis) and green fluorescent protein (GFP, x-axis), a marker of Nef-transfected cells, in control experiments. Cells transfected with empty plasmid (∆ Nef, negative control) and cells transfected with wild-type Nef plasmid (SF2 Nef, positive control) are shown. Panel B: CD4 downregulation activities of subtype A (red), B (orange), C (green) and D (blue) Nef clones are shown. Open circles identify Nef clones that displayed poor protein expression by Western blot. The solid black bar represents the median downregulation function, normalized to SF2 Nef. Panel C: Representative flow cytometry plots depict expression of surface HLA-A*02 (y-axis) and GFP (x-axis) in control experiments, as described in panel A. Panel D: HLA-I downregulation activities of subtype A (red), B (orange), C (green), and D (blue) Nef clones are shown. Open circles identify Nef clones that displayed poor expression by Western blot. The solid black bar represents the median downregulation function, normalized to SF2 Nef. Panel E: Detection of Nef clones by Western blot using rabbit anti-Nef serum. Cells transfected with control SF2 Nef, ∆Nef, and two representative patient-derived Nef clones from each subtype are shown.

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