Figure 4
From: Attenuation of multiple Nef functions in HIV-1 elite controllers
Ability of EC Nef to modulate cell surface receptor levels. Panel A: Flow cytometry plots depicting representative staining of cell-surface HLA-I (HLA-A*2402; y-axis) vs. intracellular p24Gag (x-axis) for uninfected, NL4.3-ΔNef (negative) and NL4.3-NefSF2 (positive) controls are shown. Panel B: Scatterplots depicting the ability of recombinant EC (red) and CP (blue) Nef recombinant viruses to down-regulate HLA-I are shown. Panel C: Flow cytometry plots depicting representative staining of cell-surface CD74 (y-axis) vs. intracellular p24Gag (x-axis) using uninfected and control viruses are shown. Panel D: Scatterplots depicting the ability of recombinant EC (red) and CP (blue) Nef recombinant viruses to upregulate CD74 are shown. Panel E: Flow cytometry plots depicting representative staining of cell-surface CD4 (y-axis) vs. GFP (x-axis) after delivery of no-DNA, ΔNef and NefSF2 plasmid vectors are shown. Panel F: Scatterplots depicting the ability of EC (red) and CP (blue)-derived Nef to downregulate CD4 are shown. In these experiments, results are normalized to NL4.3-NefSF2. (positive) and NL4.3-ΔNef (negative) controls. The activity of NL4.3-ΔNef or ΔNef plasmid is inherently set to zero. Bars represent median and interquartile ranges. P-values were calculated using Mann–Whitney U-test.