Figure 3
From: Attenuation of multiple Nef functions in HIV-1 elite controllers
Ability of EC Nef to enhance viral infectivity and stimulate viral replication in PBMC. Panel A: Scatterplots depict the ability of EC (red) and CP (blue) Nef recombinant viruses to enhance viral infectivity. Values are normalized to that of NL4.3-NefSF2 such that values of 100% indicate infectivity equal to that of NL4.3-NefSF2, while values <100% and >100% indicate infectivity lower than or higher than that of NL4.3-NefSF2, respectively. Bars represent median and interquartile ranges. The p-value was calculated using Mann–Whitney U-test. Panel B: Growth curves are shown for EC (red) and CP (blue) Nef recombinant viruses, plus control viruses NL4.3-NefSF2 and NL4.3-ΔNef, in PBMCs from donor #1. The mean value from quadruplicate samples is shown at each time point. The means ± SEM values for control viruses NL4.3-NefSF2 and NL4.3-ΔNef at day 9 and 12 are 115 ± 27 and 1.3 ± 0.02, and 285 ± 18.8 and 4.3 ± 0.4 respectively. Panel C: Scatterplots depict the ability of recombinant EC (red) and CP (blue) Nef recombinant viruses to stimulate viral replication in PBMC from four HIV-negative donors. Values are normalized to that of control NL4.3-NefSF2. Bars represent median and interquartile ranges. P-values were calculated using Mann–Whitney U-test.