A murine leukemia virus with Cre-LoxP excisible coding sequences allowing superinfection, transgene delivery, and generation of host genomic deletions

Table 2 Proviral expression and estimated copy numbers in GC4 subclones

Subclone	Virus	region sorted^b	Ratio of intensities	Est. # of expressed copies^a
			(GC4 EYFP)/(NIH/3T3 EYFP)	(GC4 EYFP)/(One expressed EYFP)
AYGC.2A2	Akv-Y	A	0.6	1
AYGC.2A3	Akv-Y	A	1.0	1
AYGC.2C3	Akv-Y	B	0.6	1
AYGC.3B2	Akv-Y	C	2.5	2–3
A2XYGC.2A2	Akv-2XY	D	49.8	29
A2XYGC.2B1	Akv-2XY	E	45.7	27
A2XYGC.2C1	Akv-2XY	F	34.9	21
A2XYGC.3B1	Akv-2XY	G	43.5	26
A2XYGC.3B3	Akv-2XY	G	30.1	18
A2XYGC.3B8	Akv-2XY	G	42.8	25

^a For the Akv-2XY virus, this value accounts for the difference in EYFP intensity between the unrecombined and recombined provirus. ^b Sorted regions boxed on Fig. 4.

ISSN: 1742-4690