Figure 7

Array comparative genome hybridization showing copy number changes between the mother cell line (GC4), containing Cre recombinase, and four daughter clones infected with the LoxP virus Akv-2XY; (a) A2XYGC.2B1; (b) A2XYGC.2A2; (c) A2XYGC.3B1; (d) A2XYGC.2C1. Each cell line was analyzed twice, as conventional CGH hybridizations (left) and reverse array hybridizations (right) where deletions now appear as gains. Arrows indicate regions of intrachromosomal deletion. The cell lines may or may not have a genealogical relationship in regard to LoxP virus infection. Each black dot represents the average fluorescence ratio of 4 arrayed replicates plotted as function of their position in the genome based on Celera's assembly of the mouse genomic sequence; markers contained within each BAC were blasted against the genome assembly to establish the genomic coordinate. All data have been normalized such that the median fluorescence ratio is 0. Grey vertical bars represent chromosome boundaries. A two-fold reduction in fluorescence ratio (i.e. 1 versus 2 genomic copies) is represented by a value of -1 on this scale. Since these cell lines are aneuploid, ratios are suppressed such that single copy deletions are seen as 2 versus 3 or 3 versus 4 genomic copies.