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Figure 2 | Retrovirology

Figure 2

From: Modulating HIV-1 replication by RNA interference directed against human transcription elongation factor SPT5

Figure 2

Kinetics of specific hSpt5 siRNA activity by Western blotting. HeLa cells were transfected with single-stranded antisense hSpt5 siRNA (hSpt5 (AS), lanes 1–7), hSpt5 duplex siRNA (hSpt5 (DS), lanes 8–14), or mismatch hSpt5 duplex siRNA (hSpt5-mm (DS), lanes 15–21) having 2 nucleotide mismatches between the target mRNA and the antisense strand of siRNA at the hypothetical cleavage site of the mRNA. Cells were harvested at various times post transfection. Protein content was resolved on 10% SDS-PAGE, transferred onto PVDF membranes, and immunoblotted with antibodies against hSpt5 (top bands) and hCycT1 as an internal control (lower bands).

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