Figure 1
From: Inhibition of HIV-1 replication in primary human monocytes by the IκB-αS32/36A repressor of NF-κB
Genome structure and expression of recombinant pNL(AD8)IκB-αS32/36A and pNL(AD8)IκB-antisense molecular genomes. Panel A shows the structure of pNL(AD8) derivatives that carry the IκB-αS32/36A-FLAG insert into the nef region in sense (pNL(AD8)IκB-αS32/36A) or antisense (pNL(AD8)IκB-antisense) orientations. Panel B shows the immunoblot analysis using hyperimmune AIDS patient serum of total extracts (10 μg) from 293T cells 24 hours after transfection with the indicated viral plasmids (10 μg). Panel C shows the immunoblot analysis using an anti-FLAG monoclonal antibody of total extracts (10 μg) from 293T cells 24 h after transfection with the indicated viral plasmids (10 μg).