Skip to main content

Table 2 Histochemical detection and RT-PCR amplification of Nef from human and mouse tissues

From: HIV-1 nef suppression by virally encoded microRNA

Tissues

Histochemistry*

RT-PCR†

 

F3

Anti-Nef rabbit serum

305

nef

gag

PPARγ

Human (HIV-1 uninfected)

      

Spleen

-§

-

+

-

-‡

ND

Tonsillar follicle

-

-

+

-

-

ND

Liver

-

-

+

-

-

ND

Adipose tissue (Salivary gland)

-

-

+

-

-

+

Bone marrow

-

-

+

-

-

ND

Bronchi

-

-

+

-

-

ND

Thyroid gland

-

-

+

-

-

ND

Heart muscle

-

-

+

-

-

ND

Prostate gland

-

-

+

-

-

ND

Testis

-

-

+

-

-

ND

Colon mucosa

-

-

+

-

-

ND

Lung

-

-

+

-

-

ND

Adrenal gland

-

-

+

-

-

ND

Brain (Cerebrum cortex)

-

-

+

-

-

ND

Mouse Group 1 and 2

      

Spleen

+

+

+

+

+

ND

Liver

ND

ND

+

+

+

ND

Hematopoietic cells

ND

ND

+

+

+

ND

Adipose tissue (Intestine)

+

+

+

-

-

-

Mouse Group 3

      

Spleen

-

-

+

±

±

ND

Liver

ND

ND

+

-

-

ND

Hematopoietic cells

ND

ND

+

-

-

ND

Adipose tissue (Intestine)

-

-

+

-

-

+

  1. *Histological analysis was performed with human or each group of mouse tissues by using F3 anti-Nef mAb, anti-Nef rabbit serum or 305 mAb as a primary antibody. For secondary antibody, FITC or peroxidase-conjugated antibody was used.
  2. †HIV-1 nef, PFV gag (‡HIV-1 gag for human tissues), and PPARγ mRNA expression were detected by RT-PCR with mRNA from human or each group of mouse tissues.
  3. §+, positive; -, negative; ND, not done.