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Table 2 Histochemical detection and RT-PCR amplification of Nef from human and mouse tissues

From: HIV-1 nef suppression by virally encoded microRNA

Tissues Histochemistry* RT-PCR
  F3 Anti-Nef rabbit serum 305 nef gag PPARγ
Human (HIV-1 uninfected)       
Spleen -§ - + - - ND
Tonsillar follicle - - + - - ND
Liver - - + - - ND
Adipose tissue (Salivary gland) - - + - - +
Bone marrow - - + - - ND
Bronchi - - + - - ND
Thyroid gland - - + - - ND
Heart muscle - - + - - ND
Prostate gland - - + - - ND
Testis - - + - - ND
Colon mucosa - - + - - ND
Lung - - + - - ND
Adrenal gland - - + - - ND
Brain (Cerebrum cortex) - - + - - ND
Mouse Group 1 and 2       
Spleen + + + + + ND
Liver ND ND + + + ND
Hematopoietic cells ND ND + + + ND
Adipose tissue (Intestine) + + + - - -
Mouse Group 3       
Spleen - - + ± ± ND
Liver ND ND + - - ND
Hematopoietic cells ND ND + - - ND
Adipose tissue (Intestine) - - + - - +
  1. *Histological analysis was performed with human or each group of mouse tissues by using F3 anti-Nef mAb, anti-Nef rabbit serum or 305 mAb as a primary antibody. For secondary antibody, FITC or peroxidase-conjugated antibody was used.
  2. HIV-1 nef, PFV gag (HIV-1 gag for human tissues), and PPARγ mRNA expression were detected by RT-PCR with mRNA from human or each group of mouse tissues.
  3. §+, positive; -, negative; ND, not done.