Figure 6

HTLV-1 and -2 SU subdomains interfere with infection by HTLV envelope-pseudotyped virions. 293T cells (5 × 10⁵) expressing the indicated interfering Env derivatives were infected with cell-free HTLV-2 Env-pseudotyped virions MLV(HTLV) carrying a LacZ reporter gene. Infected cells were detected 2 days later by X-gal staining. Infection values are represented as percent of control infection, i.e., relative to infection of mock (pCDNA3.1) transfected target cells, calculated as infectious units per ml of virus containing supernatant (i.u./ml). Data are representative of at least three independent experiments performed in duplicate. Error bars represent the standard error of the mean.